Neutral Buffered Formalin 10% - G-Biosciences
Dec 02, 2013 Stainsfile - 10% Neutral buffered formalin Neutral buffered formalin, usually simply shortened to NBF, has become the standard fixative for use in a diagnostic setting.It is more effective than the simple formalin mixtures as the phosphate salts present make it unlikely that erythrocytes will be damaged, and the neutral pH inhibits the formation of formalin pigment.The phosphates will adjust the pH to about 7.0 as the "neutral" infers 10 - Neutral Buffered Formalin Fixatives - Reagents A prepared 10% Neutral Phosphate Buffered Formalin is available for the histotechnologist who prefers the convenience of having a ready-to-use fixative at hand. The 10% Neutral Buffered Formalin is conveniently packaged in a five gallon cubitainer with an attached handle and easy to use "sure shut" dispenser spigot. Fixation (histology) - Wikipedia The most commonly used fixative in histology is formaldehyde. It is usually used as a 10% neutral buffered formalin (NBF), that is approx. 3.7%–4.0% formaldehyde in phosphate buffer, pH 7. Since formaldehyde is a gas at room temperature, formalin – formaldehyde gas dissolved in water (~37% w/v) – is used when making the former fixative.
Formaldehyde is CH 2 O, the simplest aldehyde. Formalin is the name for saturated (37%) formaldehyde solution. Thus, a protocol calling for 10% formalin is roughly equivalent to 4% formaldehyde. Beware though, that some solutions have methanol in them to stop polymerization but this could have a negative effect on your sample.
10% Formalin | Azer Scientific
10% Formalin | Azer Scientific
10% Neutral Buffered Formalin is a non-coagulative additive fixative; For in vitro diagnostic and laboratory use only; Optimized for histology and cytology applications *When used as a primary fixative and followed by secondary fixation by alcohol, it produces consistent histologic artifacts and results